Authors:
Daniel J. Dreyfuss, DVM, MA
Keywords:
exosomes, extracellular vesicles, cytokines, growth factors, tissue inhibitors, orthobiologics
Scientific Articles
What Are the Active Components in Hilltop Bio’s Products and How Do They Work?
- The biologically active factors in Hilltop Bio’s products include exosomes, extracellular vesicles, cytokines, growth factors and tissue inhibitors of metalloproteinases (TIMPs)
- These mediators are capable of activating the intrinsic reparative processes of damaged tissues and have demonstrated the ability to: reduce pain, scarring and inflammation; modulate angiogenesis; enhance tissue healing; possess antimicrobial properties; and possess an extracellular matrix that can serve as a scaffold for cell proliferation and differentiation
- Unlike most PRP, stem cell, and similar products, Hilltop Bio’s products offer a consistent composition for targeted treatment without variability
Regenerative medicine 2.0: extracellular vesicle–based therapeutics for musculoskeletal tissue regeneration
- Extracellular vesicles have emerged as prominent mediators of homeostasis, repair, and regeneration of musculoskeletal tissues such as bone, skeletal muscle, and cartilage
- Extracellular vesicles and exosomes as alternatives to mesenchymal stromal cells that are liable to self-replicate
- Exosomes can be more readily manipulated to display specific surface proteins for enhanced tissue targeting
- The ability to manipulate exosomes and tissue-homing properties to achieve predictable and specific biological effects can be harnessed for the development of precise and customizable therapeutics
Lyophilization of Biopharmaceuticals
- Lyophilization, or freeze-drying, is a widely used process for producing stable pharmaceutical products
- The process generally consists of three separate steps: a freezing step followed by two drying steps
- Lyophilized products can be easily transported, are stable at room temperature, and have extended shelf lives
- Lyophilized products provide many advantages to practitioners, especially those practicing in the field
Research shows efficacy in tendon injuries
This is the first study to compare the treatment of horse tendon and ligament injuries with the use of mesenchymal stromal cells (MSCs) obtained from two different sources: amniotic membrane (AMSCs) and bone marrow (BM-MSCs). The objective was to prove the ability of AMSCs to exert beneficial effects in vivo. Methods. Five million allogeneic frozen-thawed AMSCs or autologous fresh BM-MSCs were injected intralesionally in horses belonging to group A (51 horses) and group B (44 horses)…
Amniotic Tissues for the Treatment of Chronic Plantar Fasciosis and Achilles Tendinosis
Allogeneic amniotic tissue and fluid may be used to treat chronic plantar fasciosis and Achilles tendinosis. This innovative approach involves delivering a unique allograft of live human cells in a nonimmunogenic structural tissue matrix to treat chronic tendon injury. These tissues convey…
Prevention by rat amniotic fluid of adhesions after laparatomy in a rat model
The frequency of severe adhesions was 30% in the control group and 0% in the intervention group. Total adhesion scores were significantly lower in the amniotic fluid treatment group than in the control group (P 1⁄4 0.002).
Conclusions: Amniotic fluid can decrease the likelihood of post-operative intraperitoneal adhesion formation…
Effects of amniotic membrane on epithelial wound healing and stromal remodelling after excimer laser keratectomy in rabbit cornea
Use of the AM as a dressing on a corneal wound created by excimer laser surgery, in which severe haze is expected, may induce rapid epithelial healing with less inflammatory response. The AM may inhibit the irregular synthesis of stromal collagen that is associated with corneal haze…
Effect of bovine amniotic fluid on intra-abdominal adhesion in male rats
A significant reduction (P < 0.05) in adhesions formation was observed only in rats treated with male bovine amniotic fluid without cells and proteins. Therefore, BAF could be used in treatment of adhesion formation because it is inexpensive, readily available, and has minimal side effects…
Intra-articular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development
μ-dHACM is rapidly sequestered in the synovial membrane following intra-articular injection and attenuates cartilage degradation in a rat OA model. These data suggest that intra-articular delivery of μ-dHACM may have a therapeutic effect on OA development….
A Prospective Study of 20 Foot and Ankle Wounds Treated with Cryopreserved
Amniotic Membrane and Fluid Allograft
We reviewed the background information and previous clinical studies that considered the use of allogeneic amniotic tissue and fluid (granulized amniotic membrane and amniotic fluid) in the treatment of chronic diabetic foot wounds. This innovation represents a relatively new approach to wound management by delivering a unique allograft of live human cells in a non-immunogenic structural tissue matrix. Developed to fill soft tissue defects and bone voids and to convey antimicrobial and anti-inflammatory capabilities…
Cat amniotic membrane multipotent cells
are nontumorigenic and are safe for use
in cell transplantation
These findings suggest that cat amniotic membranes may be an important and useful source of mesenchymal stem cells for clinical applications, especially for cell or tissue replacement in chronic and degenerative diseases…